illumina sequencing ppt

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Illumina- Sequencing by Synthesis •Sequencing by Synthesis is the proprietary method of Illumina sequencers and involves Bridge Clonal Amplification of one of the strand after hybridisation to the flow cell surface. 0000003147 00000 n 0000057925 00000 n

It leverages sequencing by synthesis (SBS) technology – tracking the addition of labeled nucleotides as the DNA chain is copied – in a massively parallel fashion.

0000056963 00000 n 0000047051 00000 n Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used. 0000030798 00000 n %%EOF h�b```f``�c`c``�� € "@1v�������'|��p��(}R�!v��u�< ���=~�u��%�o�x̀0J�D?[ڂf���R:.�pdqJBCw��ϙOp�d:-�a�q���N�R.+�e^T2��3�:9��t��]�`I���W��=�:���7�O2����_���"��Jz=��5}��j8.

0000002187 00000 n Introduction • DNA - the hereditary material written in four-letter code of nucleotides. • sequencing, or "reading" the genetic code has … :1I���̗��[J����� {w^=y��%V��>��A���M�TZy��lf�^��r�k�4��Y>y�F�\��jQ�H�[�n��=�Ԝ� �ͮE�N6� �OF��+P��l���/��87~��Z�ӱ�1'�R��;—'/c2�ؤ�jn5Ga�cL=&�N6y�����Z6)�8�b4�Rrc�E%�+��N���5�'+�P;~J���$��o���4(�h F��khE4�����T��0 Illumina sequencing utilizes a fundamentally different approach from the classic Sanger chain-termination method. 0000045058 00000 n 0000739726 00000 n �� i+TR E6 �]����KhXZ:�2�~%�� If you continue browsing the site, you agree to the use of cookies on this website. 0000057540 00000 n

0000044339 00000 n 0 See our (���נ��.D`d�#�M� f�>XC�LR�WJ�p��}��Ql��.�!i�-5�!S���w�Ϗ���8����-L��~��{��c-p�x:Y(���bB�W�59��Z��1�1�>kZ{+�>D��j�J=�jia��0���`bi[��[�vO�"�><>GB�]^x]9]�\s����`�}[�$����a����-ty�2������g�� ��Gn�\�V���YC�&����{�S This presentation covers how Illumina Sequencing, a type of Next Generational Sequencing, works. 0000075252 00000 n 0000111055 00000 n 0000057347 00000 n

Looks like you’ve clipped this slide to sequence at lower resolution, but process larger sample numbers to achieve greater statistical power within a given population of interest.

@� � ��GH 0000079978 00000 n •Hybridisation takes place with the help of one of the adaptors attached to the template.

Gene sequencing methods 1. 0000075174 00000 n %PDF-1.4 %���� 1831 M. Pharm. 0000058117 00000 n 0000075018 00000 n If you continue browsing the site, you agree to the use of cookies on this website. 0000099757 00000 n Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.

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0000003385 00000 n H��W�nc���+��棞�`�FY� ���3�;�L��sHY֕ں#�n���o�>HV�:������鿿~�|���?���������}�as��������?|��Nܴ��xBR������_6�¯)Ę7��[��s��������͇����ʹv3��,k7i�&�ݔ������J�����N��/-��+����]� �us��e��ݻJI�#n�Ls�'�>��.��{��0?�-X���wA Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. �#�

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